Home Research Project Details B1 - Spatio-temporal coding and imprinting in the Drosophila olfactory system
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B1 - Spatio-temporal coding and imprinting in the Drosophila olfactory system

André Fiala, Annette Witt, and Marc Timme

Odour processing by neuronal circuits is complex, highly multidimensional, and transient. Odours activate or inhibit a diverse array of olfactory receptor neurons, thereby inducing dynamical processes in downstream circuits. In Drosophila, such sensory signals affect a neuronal network within the antennal lobe where odours are represented by dynamically interacting neurons of three different types: sensory neurons (SNs), excitatory and inhibitory local interneurons (eLIs, iLIs), and projection neurons (PNs). So far, it is unclear how SNs, LIs, and PNs cooperatively interact to support odor distinction and odour classification. Drosophila provides an ideal model system for studying spatiotemporal olfactory processing, because subpopulations of neurons can be genetically targeted. This project aims at describing, quantifying and modelling how the various parts of the primary olfactory circuitry contribute to spatio-temporal odour representation and how their dynamics directly relate to computations and resulting behaviours. In particular, we are going to investigate the following problems:

(A) Finding a quantitative description of odour representation in the antennal lobe,

(B) Modifying odor representations by imprinting the animals (i.e., a continuous exposure of Drosophila to odorants and odor combinations during a critical time window),

(C) Modelling the mode of action of olfactory neurons, in particular in LIs, in terms of recurrent neural circuit models.

In summary, this study will help to dissect the contributions of various neuronal subpopulations for odour coding and thus provide important functional information for both odour coding and olfactory imprinting in flies.

Belongs to Group(s):
Network Dynamics, Molecular Neurobiology of Behaviour

Is part of  Section B 

Members working within this Project:
Shubham, Dipt 
Witt, Annette 
Fiala, André 

Selected Publication(s):

Barth, J, Dipt, S, Pech, U, Hermann, M, Riemensperger, T, and Fiala, A (2014).
Differential Associative Training Enhances Olfactory Acuity in Drosophila melanogaster
The Journal of Neuroscience 34(5):1819-1837.

Dipt, S, Riemensperger, T, and Fiala, A (2014).
Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster
Methods in Molecular Biology 1071:195-206.

Pech, U, Revelo, NH, Seitz, KJ, Rizzoli, SO, and Fiala, A (2014).
Optical Dissection of Experience-Dependent Pre- and Postsynaptic Plasticity in the Drosophila Brain
Cell Reports 10(12):2083-2095.

Vasmer, D, Pooryasin, A, Riemensperger, T, and Fiala, A (2014).
Induction of aversive learning through thermogenetic activation of Kenyon cell ensembles in Drosophila
Frontiers in Behavioral Neuroscience 8(Article 174):1-14.

Gupta, VK, Scheunemann, L, Eisenberg, T, Mertel, S, Bhukel, A, Koemans, TS, Kramer, JM, Liu, KS, Schroeder, S, Stunnenberg, HG, Sinner, F, Magnes, C, Pieber, TR, Dipt, S, Fiala, A, Schenck, A, Schwaerzel, M, Madeo, F, and Sigrist, SJ (2013).
Restoring polyamines protects from age-induced memory impairment in an autophagy-dependent manner
Nature Neuroscience 16(10):1453-1460.

Pech, U, Dipt, S, Barth, J, Singh, P, Jauch, M, Thum, AS, Fiala, A, and Riemensperger, T (2013).
Mushroom body miscellanea: transgenic Drosophila strains expressing anatomical and physiological sensor proteins in Kenyon cells
Front Neural Circuits 7(Article 147):1-14.

Pech, U, Pooryasin, A, Birman, S, and Fiala, A (2013).
Localization of the Contacts Between Kenyon Cells and Aminergic Neurons in the Drosophila melanogaster Brain Using SplitGFP Reconstitution
The Journal of Comparative Neurology 521:3992-4026.

Riemensperger, T, Pech, U, Dipt, S, and Fiala, A (2012).
Optical calcium imaging in the nervous system of Drosophila melanogaster
Biochim Biophys Acta 1820(8):1169-1178.

Niewalda, T, Völler, T, Eschbach, C, Ehmer, J, Chou, WC, Timme, M, Fiala, A, and Gerber, BA (2011).
A Combined Perceptual, Physico-Chemical, and Imaging Approach to 'Odour-Distances' Suggests a Categorizing Function of the Drosophila Antennal Lobe
PLoS One 6(9):e24300.